College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan, China.
J Chromatogr A. 2011 Oct 14;1218(41):7341-6. doi: 10.1016/j.chroma.2011.08.045. Epub 2011 Aug 19.
A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50 μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples.
基于适体的吸附剂被固定在磁性纳米球（MNS）上，并用于开发一种磁固相萃取程序，与高效液相色谱分离和荧光检测结合使用，以净化食品样品。吸附剂对赭曲霉毒素 A 的特异性保留得到了证明，并确定了 MNS-适体吸附剂的容量。通过与商用 C18 柱的固相萃取进行比较，成功评估了这种新方法的效果。几种不同的食品样品在 2.5-50μg/kg 的范围内进行了加标，平均回收率分别为 67%-90%。最后，该寡聚糖吸附剂被应用于从未加标食品样品中选择性提取赭曲霉毒素 A。
