猪圆环病毒 2 型（PCV2）2a 基因型和 2b 基因型重组突变体在体外表现出明显增强的病毒复制能力和改变的抗原性。
Porcine circovirus genotype 2a (PCV2a) and genotype 2b (PCV2b) recombinant mutants showed significantly enhanced viral replication and altered antigenicity in vitro.
机构信息
Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
出版信息
Virology. 2011 Oct 25;419(2):57-63. doi: 10.1016/j.virol.2011.08.004. Epub 2011 Sep 3.
Two recombinant mutants of porcine circovirus type 2 (PCV2), which resulted from replacement of a genomic fragment containing the open reading frame 2 (ORF2) of genotype PCV2b with that of genotype PCV2a, were obtained initially from co-infection with PCV2a and 2b genotype viruses in vitro. The two mutant viruses contained the ORF1 sequence from genotype PCV2b and the ORF2 sequence from genotype PCV2a. They were designated according to the nomenclature proposed by Grau et al., indicating the origin of the ORF1 sequence first and that of the ORF2 sequence second, i.e., PCV2b(JF11)/2a(CL1) and PCV2b(YJ)/2a(CL1). The replication efficiencies of the two PCV2 recombinant mutants were enhanced significantly and their antigenicities were altered significantly in vitro when compared with their parental strains.
最初从 PCV2a 和 2b 基因型病毒的体外共感染中获得了两种猪圆环病毒 2 型（PCV2）的重组突变体，这两种突变体是通过替换包含基因型 PCV2b 的开放阅读框 2（ORF2）的基因组片段而产生的。这两种突变病毒含有来自基因型 PCV2b 的 ORF1 序列和来自基因型 PCV2a 的 ORF2 序列。它们是根据 Grau 等人提出的命名法命名的，首先表示 ORF1 序列的来源，其次表示 ORF2 序列的来源，即 PCV2b(JF11)/2a(CL1)和 PCV2b(YJ)/2a(CL1)。与亲本株相比，这两种 PCV2 重组突变体的复制效率显著提高，其体外抗原性也发生了显著改变。
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