Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Bioresour Technol. 2011 Nov;102(21):9852-9. doi: 10.1016/j.biortech.2011.07.108. Epub 2011 Aug 11.
The azoreductase PpAzoR from Pseudomonas putida shows a broader specificity for decolourization of azo dyes than CotA-laccase from Bacillus subtilis. However, the final products of PpAzoR activity exhibited in most cases a 2 to 3-fold higher toxicity than intact dyes themselves. We show that addition of CotA-laccase to PpAzoR reaction mixtures lead to a significant drop in the final toxicity. A sequential enzymatic process was validated through the use of 18 representative azo dyes and three model wastewaters that mimic real dye-containing effluents. A heterologous Escherichia coli strain was successfully constructed co-expressing the genes coding for both PpAzoR and CotA. Whole-cell assays of recombinant strain for the treatment of model dye wastewater resulted in decolourization levels above 80% and detoxification levels up to 50%. The high attributes of this strain, make it a promising candidate for the biological treatment of industrial dye containing effluents.
恶臭假单胞菌中的芳香胺还原酶 PpAzoR 比枯草芽孢杆菌中的 CotA-漆酶对偶氮染料的脱色具有更广泛的特异性。然而，PpAzoR 活性的最终产物在大多数情况下表现出比完整染料本身高 2 到 3 倍的毒性。我们表明，向 PpAzoR 反应混合物中添加 CotA-漆酶会导致最终毒性显著降低。通过使用 18 种代表性偶氮染料和三种模拟实际含染料废水的模型废水，验证了顺序酶促过程。成功构建了共表达编码 PpAzoR 和 CotA 的基因的异源大肠杆菌菌株。重组菌株对模型染料废水的全细胞测定导致脱色水平超过 80%，解毒水平高达 50%。该菌株的高属性使其成为处理工业含染料废水的有前途的生物处理候选物。
