Environmental Biotechnology Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Korean Collection for Type Cultures, Biological Resource Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1674-1678. doi: 10.1099/ijs.0.034868-0. Epub 2011 Sep 2.
A Gram-negative, aerobic, motile and rod-shaped bacterium, designated strain T7-07(T), was isolated from compost in Daejeon, Korea. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain T7-07(T) had 99.0% gene sequence similarity with Arenimonas malthae KACC 14618(T) and 94.7-95.9% with other recognized species of the genus Arenimonas. Cells formed creamy white to yellowish colonies on R2A agar and contained Q-8 as the predominant ubiquinone, C(15:0) iso, C(16:0) iso, C(17:1) iso ω9c and C(11:0) iso 3-OH as the major fatty acids, and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine and an unknown aminolipid as the major polar lipids. The DNA G+C content of strain T7-07(T) was 68.3 mol%. DNA-DNA reassociation experiments between T7-07(T) and Arenimonas malthae KACC 14618(T) resulted in a mean relatedness value of 22.2%. Combined genotypic and phenotypic data supported the conclusion that the strain T7-07(T) represents a novel species, for which the name Arenimonas daejeonensis sp. nov. is proposed. The type strain is T7-07(T) (=KCTC 12667(T)=DSM 18060(T)).
一株革兰氏阴性、需氧、能运动的杆菌，命名为 T7-07(T) 菌株，从韩国大田的堆肥中分离得到。基于 16S rRNA 基因测序的系统发育分析表明，T7-07(T) 菌株与 Arenimonas malthae KACC 14618(T) 的基因序列相似性为 99.0%，与其他公认的 Arenimonas 属种的相似性为 94.7-95.9%。细胞在 R2A 琼脂上形成奶油色至黄色菌落，含有 Q-8 作为主要的泛醌，C(15:0) iso、C(16:0) iso、C(17:1) iso ω9c 和 C(11:0) iso 3-OH 作为主要的脂肪酸，以及双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰甲乙醇胺和一种未知的氨基脂作为主要的极性脂。T7-07(T) 菌株的 DNA G+C 含量为 68.3 mol%。T7-07(T) 和 Arenimonas malthae KACC 14618(T) 之间的 DNA-DNA 同源性实验产生了 22.2%的平均相关值。综合基因型和表型数据支持该菌株 T7-07(T) 代表一个新种的结论，因此建议将其命名为 Arenimonas daejeonensis sp. nov.。模式菌株为 T7-07(T) (=KCTC 12667(T)=DSM 18060(T))。
