Askanas V, Bornemann A, Engel W K
Department of Neurology, University of Southern California School of Medicine, Los Angeles.
Neurology. 1990 Jun;40(6):949-53. doi: 10.1212/wnl.40.6.949.
We localized desmin at human neuromuscular junctions (NMJs) using specific anti-desmin monoclonal and polyclonal antibodies. It was highly concentrated in the postsynaptic domain. It was found in all the NMJs examined. Compared with the area of acetylcholine receptors identified by bound alpha-bungarotoxin, the strong desmin immunoreactivity occupied a slightly larger area and was localized slightly deeper in the muscle fiber, suggesting that desmin might be accumulated within the postsynaptic folds and possibly below the folds. This appears to be the first identification of a protein unrelated to cholinergic transmission accumulated at human NMJs.
我们使用特异性抗结蛋白单克隆抗体和多克隆抗体，将结蛋白定位在人类神经肌肉接头（NMJ）处。它高度集中于突触后区域。在所检查的所有神经肌肉接头中均发现了它。与由结合的α-银环蛇毒素识别出的乙酰胆碱受体区域相比，强烈的结蛋白免疫反应性占据的区域略大，且在肌纤维中的定位略深，这表明结蛋白可能积聚在突触后褶皱内以及可能在褶皱下方。这似乎是首次鉴定出在人类神经肌肉接头上积聚的与胆碱能传递无关的蛋白质。
