Kai O, Fujioka T, Yasuda M
Histochemistry. 1979 Feb 21;59(4):305-10. doi: 10.1007/BF00689612.
Two method for isolating the intramitochondrial bodies from bovine adrenocortical cells are proposed. Electron microscopic examination shows that discontinuous sucrose density gradient centrifugation can separate the fraction rich in intramitochondrial bodies, but some indistinguishable fragments remain among them. Continuous sucrose density gradient centrifugation is probably superior to the former method in obtaining a highly purified fraction of the bodies. The amido black positive granules, presumed to be intramitochondrial bodies, are collected in the fractions of the sucrose density of around 1.27 (1.23--1.30), which lack cytochrome c oxidase activity.
提出了两种从牛肾上腺皮质细胞中分离线粒体内体的方法。电子显微镜检查表明，不连续蔗糖密度梯度离心可以分离出富含线粒体内体的部分，但其中仍有一些难以区分的碎片。在获得高度纯化的线粒体内体部分方面，连续蔗糖密度梯度离心可能优于前一种方法。推测为线粒体内体的氨基黑阳性颗粒收集在蔗糖密度约为1.27（1.23 - 1.30）的部分中，这些部分缺乏细胞色素c氧化酶活性。
