RAHMAN Y E
J Cell Biol. 1962 May;13(2):253-60. doi: 10.1083/jcb.13.2.253.
Fractions from rat thymuses were isolated by sucrose density-gradient centrifugation, before and after 1000 r whole-body x-irradiation, and examined by electron microscopy. Cytochrome oxidase and acid phosphatase activities of these fractions were tested as well. Electron-opaque bodies with diameters ranging from 0.10 to 0.35 micro, with a mean of 0.25 micro, were found in fractions having high acid phosphatase activity, while the fractions rich in cytochrome oxidase consisted mostly of mitochondria. After irradiation, there was an increased ratio of dense bodies to mitochondria. These particles are considered to be lysosomes similar to those identified in other rat tissues. Their relationship to the mitochondria is discussed.
在1000伦琴全身X射线照射前后,通过蔗糖密度梯度离心法分离大鼠胸腺的各组分,并进行电子显微镜检查。还检测了这些组分的细胞色素氧化酶和酸性磷酸酶活性。在具有高酸性磷酸酶活性的组分中发现了直径范围为0.10至0.35微米、平均为0.25微米的电子不透明体,而富含细胞色素氧化酶的组分主要由线粒体组成。照射后,致密体与线粒体的比例增加。这些颗粒被认为是与在其他大鼠组织中鉴定出的溶酶体相似的溶酶体。讨论了它们与线粒体的关系。