Sanchís-Bayarri Vaillant V, Fraile Fariñas M T, Chanza Aviño M, Sanchís-Bayarri Bernal V, Aguirre Cepeda F
Sección de Virología, Hospital General, Valencia.
An Esp Pediatr. 1990 Mar;32(3):219-21.
We study 18 children with acute respiratory infection diagnosed early by direct immunofluorescence (DIF) using monoclonal antibodies to synticial respiratory virus (SRV). Antibodies to adenovirus, influenza virus A and B, parainfluenza virus 1, 2 and 3, mycoplasma, Q fever, herpes and cytomegalovirus (CMV) are also studied. We consider that in most cases the diagnosis of infections caused by SRV cannot be established by complement fixation, and should be accompanied by some antigen detection technique. Direct immunofluorescence facilitates the early detection of SRV in most cases before the appearance of antibodies to this virus. Only one of the 18 patients with positive DIF presented antibodies to SRV.
我们研究了18名通过直接免疫荧光法（DIF）早期诊断为急性呼吸道感染的儿童，该方法使用针对合成呼吸道病毒（SRV）的单克隆抗体。同时也研究了针对腺病毒、甲型和乙型流感病毒、副流感病毒1、2和3、支原体、Q热、疱疹病毒和巨细胞病毒（CMV）的抗体。我们认为，在大多数情况下，不能通过补体结合试验来确诊由SRV引起的感染，而应辅以某种抗原检测技术。直接免疫荧光法有助于在大多数情况下，在针对该病毒的抗体出现之前早期检测出SRV。18例DIF阳性的患者中只有1例出现了针对SRV的抗体。
