单纯疱疹病毒 1 型基因组的克隆作为一种新型荧光素酶标记的传染性细菌人工染色体。

Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome.

机构信息

Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuchang, People's Republic of China.

出版信息

Arch Virol. 2011 Dec;156(12):2267-72. doi: 10.1007/s00705-011-1094-9. Epub 2011 Sep 6.

DOI:10.1007/s00705-011-1094-9

PMID:21894520

Abstract

Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.

摘要

单纯疱疹病毒 1 型（HSV-1）是一种普遍存在于皮肤和黏膜的人类病原体。在本研究中，我们将 HSV-1 F 株的基因组克隆为无病毒基因缺失的感染性细菌人工染色体（BAC）克隆。此外，我们还插入了萤火虫荧光素酶盒，以生成一种新型的表达荧光素酶的 HSV-1 BAC。重要的是，所得的重组 HSV-1 BAC Luc 在 Vero 细胞中与野生型病毒表现一致，并且可以在体外轻松定量荧光素酶活性。因此，这种新型的 HSV-1 BAC 系统将成为基因功能分析的有力工具。