Huo Su-Xin, Zhu Yin-Chu, Chen Liu, Yun Tao, Ye Wei-Cheng, Hua Jiong-Gang, Ni Zheng, Xiang Sheng-Rui, Ding Fang-Zhou, Gao Xu, Liu Han-Bin, Bao En-Dong, Zhang Cun
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Transbound Emerg Dis. 2024 May 17;2024:1746963. doi: 10.1155/2024/1746963. eCollection 2024.
In 2021, a highly virulent strain of duck enteritis virus (DEV), designated as DEV XJ, was isolated from Zhejiang, China, and its complete genome, spanning 162,234 bp with 78 predicted open reading frames (ORFs), was sequenced. While showing relative homology to the DEV CV strain, DEV XJ exhibited distinctions in 38 ORFs, including various immunogenic and virulence-related genes. Amino acid variation analysis, focusing on and , indicated a high degree of homology between DEV XJ and the 2085 strain from Europe, as well as the DEV DP-AS-Km-19 strain from India. Subsequently, a full-length infectious bacterial artificial chromosome clone (BAC) of DEV XJ was successfully constructed to delve into the pathogenic mechanisms of this virulent strain. XJ BAC demonstrated substantial similarity to the parental DEV XJ in both growth properties and the induction of typical pathogenic symptoms in sheldrakes. Furthermore, the , , , and genes were individually deleted using a two-step RED recombination approach based on the infectious BAC clone. Our findings revealed that the and genes play crucial roles in viral proliferation. Although the and genes were dispensable for viral replication and cell-to-cell transmission , they attenuated the replication and transmission efficiency of DEV compared to the WT. In summary, this study accomplished the whole-genome sequencing of a clinically virulent DEV strain and the successful construction of an infectious DEV XJ clone. Moreover, the functional roles of the above-mentioned mutant genes were preliminarily explored through the analysis of their biological characteristics.
2021年，从中国浙江分离出一种高毒力鸭肠炎病毒（DEV）毒株，命名为DEV XJ，并对其全长162234 bp、包含78个预测开放阅读框（ORF）的完整基因组进行了测序。虽然DEV XJ与DEV CV毒株具有相对同源性，但在38个ORF中表现出差异，包括各种免疫原性和毒力相关基因。对 和 的氨基酸变异分析表明，DEV XJ与来自欧洲的2085毒株以及来自印度的DEV DP-AS-Km-19毒株之间具有高度同源性。随后，成功构建了DEV XJ的全长感染性细菌人工染色体克隆（BAC），以深入研究这种毒力毒株的致病机制。XJ BAC在生长特性和诱导麻鸭出现典型致病症状方面与亲本DEV XJ表现出高度相似性。此外，基于感染性BAC克隆，采用两步RED重组方法分别缺失了 、 、 和 基因。我们的研究结果表明， 和 基因在病毒增殖中起关键作用。虽然 和 基因对于病毒复制和细胞间传播是可有可无的，但与野生型相比，它们降低了DEV的复制和传播效率。总之，本研究完成了临床毒力DEV毒株的全基因组测序，并成功构建了感染性DEV XJ克隆。此外，通过分析上述突变基因的生物学特性，初步探索了它们的功能作用。
