Institute of Ecology and Evolutionary Biology, National Taiwan University, Rm 1227, Life Science Building, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
J Plant Res. 2012 May;125(3):317-26. doi: 10.1007/s10265-011-0447-5. Epub 2011 Sep 6.
Balanophora japonica and B. yakushimensis are two putatively agamospermic taxa previously reported from southern Japan. Their inflorescences superficially represent those of B. laxiflora and B. fungosa. In this study we confirmed their presence in Taiwan by morphological and phylogenetic analysis using nuclear 18S rDNA and nrITS sequences with related taxa. B. japonica, B. yakushimensis, and B. laxiflora formed a well-supported clade that is distinct from other Balanophora. All three taxa also show considerable differences on morphological and nucleotide sequence differences, therefore the name of B. yakushimensis is retained. The results provide new insights on the intrageneric classification of Balanophora and suggest the positioning of female flowers should be down-weighted. We also successfully identify the hosts of B. japonica and B. yakushimensis by amplifying chloroplast matK sequences from the connected root tissues. The results showed that B. japonica parasitizes on Symplocos species, and that B. yakushimensis parasitizes on Distylium racemosum in Japan and Schima superba in Taiwan's population.
日本阴地蕨和雅苏阴地蕨是两个先前报道于日本南部的可能无融合生殖类群。它们的花序在表面上与日本阴地蕨和鳞毛阴地蕨的相似。在这项研究中，我们通过使用核 18S rDNA 和 nrITS 序列与相关类群进行形态和系统发育分析，在台湾确认了它们的存在。日本阴地蕨、雅苏阴地蕨和日本阴地蕨形成了一个与其他阴地蕨明显不同的支持良好的分支。这三个类群在形态和核苷酸序列上也表现出相当大的差异，因此保留了雅苏阴地蕨的名称。研究结果为阴地蕨属的种内分类提供了新的见解，并表明应降低雌花的权重。我们还通过从连接的根组织中扩增叶绿体 matK 序列，成功鉴定了日本阴地蕨和雅苏阴地蕨的宿主。结果表明，日本阴地蕨寄生在 Symplocos 属上，而雅苏阴地蕨在日本寄生在 Distylium racemosum 上，在台湾的种群中寄生在杨桐上。
