Department of Internal Medicine III, University Hospital, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
Neoplasma. 2011;58(6):499-506.
The aim of the study was to analyze differences in the serum levels of 8 selected biological parameters between monoclonal gammopathy of undetermined significance (MGUS) and different stages of multiple myeloma (MM), potentially beneficial for distinguishing between the two conditions. The analyzed group of 131 subjects comprised 62 individuals with MGUS and 69 MM patients examined at the time of diagnosis. The serum levels were determined by a quantitative immunoradiometric assay (insulin-like growth factor 1, IGF-1) and quantitative sandwich enzyme immunoassay (osteopontin, OPN; endostatin, ES; macrophage inflammatory proteins 1α/β, MIP-1α/β; angiogenin, ANG; and interleukin 17, IL-17). The analysis showed a statistically significant difference in serum concentrations between MGUS and the symptomatic form of MM using the Durie-Salmon (D-S) staging system only in the cases of OPN and stages II and III (0.001 and MM. More benefit may be expected from analyses using multiparametric immunophenotyping of plasma cells and molecular biology methods including gene expression analysis and proteomics.
本研究旨在分析血清中 8 种选定的生物学参数水平在意义未明的单克隆丙种球蛋白血症（MGUS）和多发性骨髓瘤（MM）不同阶段之间的差异，这些差异可能有助于区分这两种情况。在这项研究中，分析了 131 名受试者，其中 62 名患有 MGUS，69 名 MM 患者在诊断时接受了检查。血清水平通过定量免疫放射测定法（胰岛素样生长因子 1，IGF-1）和定量夹心酶免疫分析法（骨桥蛋白，OPN；内皮抑素，ES；巨噬细胞炎性蛋白 1α/β，MIP-1α/β；血管生成素，ANG；白细胞介素 17，IL-17）进行测定。分析显示，仅在用 Durie-Salmon（D-S）分期系统对 MM 进行分期时，MGUS 和有症状形式的 MM 之间的血清浓度存在统计学显著差异，OPN 和 II 期和 III 期（0.001 和 MM。通过对血浆细胞的多参数免疫表型分析以及包括基因表达分析和蛋白质组学在内的分子生物学方法进行分析，可能会获得更多益处。
