Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Cancer Sci. 2011 Dec;102(12):2172-8. doi: 10.1111/j.1349-7006.2011.02089.x. Epub 2011 Oct 18.
The expression of Rab proteins has been associated with cancer. However, few data are available on Rab5A expression in human breast cancer or its impact on disease progression. First, we examined the functional role of Rab5A in breast cancer cells. The expression of Rab5A in MDA-MB-231 cells can be stimulated by epidermal growth factor in a dose-dependent manner. The epidermal growth factor-induced increase of Rab5A expression correlated well with enhanced migration in wound healing migration assays in these cells. Furthermore, we evaluated the expression of Rab5A in breast cancer specimens using immunohistochemical staining, then analyzed the relationship between the expression of Rab5A and clinicopathological parameters. The increased expression of Rab5A protein in 123 breast cancer samples was associated with higher histological grade (P = 0.004), more lymphovascular invasion (P = 0.027), more axillary lymph node (LN) metastasis (P = 0.008), and a higher number of axillary LN metastases (P = 0.043). Among 218 axillary LNs of more than 10 breast cancer patients with node metastases, 167 metastatic LNs were found to have increased Rab5A expression. Rab5A is associated with axillary LN metastasis in breast cancer patients.
Rab 蛋白的表达与癌症有关。然而，关于 Rab5A 在人乳腺癌中的表达及其对疾病进展的影响的数据很少。首先，我们研究了 Rab5A 在乳腺癌细胞中的功能作用。表皮生长因子可以以剂量依赖的方式刺激 MDA-MB-231 细胞中 Rab5A 的表达。表皮生长因子诱导的 Rab5A 表达增加与这些细胞中划痕愈合迁移实验中的迁移增强密切相关。此外，我们使用免疫组织化学染色评估了 Rab5A 在乳腺癌标本中的表达，然后分析了 Rab5A 表达与临床病理参数之间的关系。在 123 例乳腺癌样本中，Rab5A 蛋白的表达增加与组织学分级较高（P = 0.004）、淋巴管血管侵犯更多（P = 0.027）、腋窝淋巴结（LN）转移更多（P = 0.008）以及腋窝 LN 转移数量更多（P = 0.043）有关。在 218 例腋窝 LN 中有超过 10 例乳腺癌患者有淋巴结转移，其中 167 例转移性 LN 发现 Rab5A 表达增加。Rab5A 与乳腺癌患者的腋窝 LN 转移有关。
