Sriwichai C, Jiraudommongkol M, Wutti-In Y, Vongchan P
The Small Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Small Anim Pract. 2011 Sep;52(9):476-83. doi: 10.1111/j.1748-5827.2011.01103.x.
Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells. Commercial reagents are available, but these often differ in sensitivity and specificity. Rabbit anticanine globulins (polyspecific) were produced for use in canine blood compatibility testing and in the investigation of immune-mediated haemolytic anaemia.
Canine sera was pooled, IgG was purified and subsequently used to immunise rabbits. A rising titre of anticanine IgG was demonstrated by indirect enzyme-linked immunosorbent assay. Rabbit anticanine complement was isolated and investigated by agglutination of complement-coated canine red blood cells. Both antibodies were purified and checked for crossreactivity before being combined to polyspecific anticanine globulins. The obtained reagent was used to indicate sensitised canine red blood cells and free antibodies in three different types of clinical samples, including blood for compatibility testing and that for investigation of immune-mediated haemolytic anaemia and screening for post-transfusion alloantibodies and was also compared to commercial Coombs' reagent.
The product provided results in accordance with those from commercial Coombs' reagent. The sensitivity for canine crossmatching was 100% and specificity for diagnosing immune-mediated haemolytic anaemia was 87%.
This product is helpful for canine crossmatching purposes and in the investigation of immune-mediated haemolytic anaemia.
犬同种或自身抗体在临床上具有重要意义，但这些免疫球蛋白G（IgG）抗体的附着不会产生可观察到的血凝反应。需要犬球蛋白抗体来证明红细胞的致敏情况。有商业试剂可供使用，但这些试剂的敏感性和特异性往往存在差异。制备兔抗犬球蛋白（多特异性）用于犬血液相容性检测和免疫介导的溶血性贫血的研究。
收集犬血清，纯化IgG，随后用于免疫兔子。通过间接酶联免疫吸附测定法证明抗犬IgG滴度上升。分离兔抗犬补体，并通过补体包被的犬红细胞凝集进行研究。在将两种抗体合并为多特异性抗犬球蛋白之前，对其进行纯化并检查交叉反应性。将获得的试剂用于指示三种不同类型临床样本中致敏的犬红细胞和游离抗体，包括用于相容性检测的血液、用于免疫介导的溶血性贫血研究的血液以及用于输血后同种抗体筛查的血液，并与商业库姆斯试剂进行比较。
该产品提供的结果与商业库姆斯试剂的结果一致。犬交叉配血的敏感性为100%，诊断免疫介导的溶血性贫血的特异性为87%。
该产品有助于犬交叉配血以及免疫介导的溶血性贫血的研究。
