Tomochika Keisuke, Shimizu-Ibuka Akiko, Tamura Tomoko, Mura Kiyoshi, Abe Naoki, Onose Jun-Ichi, Arai Soichi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture.
Biosci Biotechnol Biochem. 2011;75(9):1644-8. doi: 10.1271/bbb.110085. Epub 2011 Sep 7.
Peanut skin contains large amounts of polyphenols having antiallergic effects. We found that a peanut-skin extract (PSE) inhibits the degranulation induced by antigen stimulation of rat basophilic leukemia (RBL-2H3) cells. A low-molecular-weight fraction from PSE, PSEL, also had inhibitory activity against allergic degranulation. A main polyphenol in PSEL was purified by gel chromatography and fractionated by YMC-gel ODS-AQ 120S50 column. Electrospray ionization mass spectrometry (ESI-MS) analysis of the purified polyphenol gave m/z 599 [M+Na]⁺. Based on the results of ¹H-NMR, ¹³C-NMR spectra, and optical rotation analysis, the polyphenol was identified as procyanidin A1. It inhibited the degranulation caused by antigen stimulation at the IC₅₀ of 20.3 µM. Phorbol-12-myristate-13-acetate (PMA) and 2,5,-di(tert-butyl)-1,4-hydroquinone (DTBHQ)-induced processes of degranulation were also inhibited by procyanidin A1. These results indicate that peanut-skin procyanidin A1 inhibits degranulation downstream of protein kinase C activation or Ca²⁺ influx from an internal store in RBL-2H3 cells.
花生皮含有大量具有抗过敏作用的多酚。我们发现花生皮提取物（PSE）可抑制大鼠嗜碱性白血病（RBL-2H3）细胞经抗原刺激诱导的脱颗粒。PSE中的低分子量组分PSEL对过敏性脱颗粒也具有抑制活性。通过凝胶色谱法纯化PSEL中的一种主要多酚，并通过YMC凝胶ODS-AQ 120S50柱进行分离。对纯化后的多酚进行电喷雾电离质谱（ESI-MS）分析，得到m/z 599 [M+Na]⁺。基于¹H-NMR、¹³C-NMR光谱结果以及旋光分析，该多酚被鉴定为原花青素A1。它在IC₅₀为20.3 µM时抑制由抗原刺激引起的脱颗粒。原花青素A1还抑制佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）和2,5-二（叔丁基）-1,4-对苯二酚（DTBHQ）诱导的脱颗粒过程。这些结果表明，花生皮原花青素A1在RBL-2H3细胞中抑制蛋白激酶C激活或从内部储存库流入Ca²⁺下游的脱颗粒。
