Sato Takahiro, Akiyama Masako, Nakahama Ken-Ichi, Seo Shujiro, Watanabe Masamichi, Tatsuzaki Jin, Morita Ikuo
Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Functional and Phytochemical Laboratory, Tokiwa Phytochemical Co., Ltd., 158 Kinoko, Sakura-shi, Chiba, 285-0801, Japan.
J Nat Med. 2018 Jan;72(1):211-219. doi: 10.1007/s11418-017-1135-0. Epub 2017 Oct 10.
We report in this study novel biochemical activities of peanut skin extract (PEXT) on thrombocytopoiesis. Peanut skin, derived from Arachis hypogaea L., is a traditional Chinese medicine that is used to treat chronic hemorrhage. We have shown that oral administration of PEXT increases the peripheral platelet levels in mice. Recently, we reported a liquid culture system that is useful for investigating megakaryocytopoiesis and thrombocytopoiesis from human CD34 cells. In this liquid culture system, PEXT was shown to enhance the formation of CD41/DAPI cells (platelets), but had no effect on the formation of CD41/DAPI cells (megakaryocytes) or on the DNA content. Furthermore, PEXT selectively stimulated proplatelet formation from cultured mature megakaryocytes and phorbol 12-myristate 13 acetate (PMA)-induced formation of platelet-like particles from Meg01 cells. Despite having no influence on the formation of megakaryocyte colony forming units (CFUs), PEXT increased the size of megakaryocytes during their development from CD34 cells. PEXT showed no effect on the GATA-1 and NF-E2 mRNA levels, which are known to play an important role in thrombocytopoiesis and, based on the results of a pMARE-Luc (pGL3-MARE-luciferase) assay, had no influence on NF-E2 activation in Meg01 cells. These results suggest that PEXT accelerates proplatelet formation from megakaryocytes but does not influence the development of hematopoietic stem cells into megakaryocytes.
我们在本研究中报告了花生皮提取物（PEXT）在血小板生成方面的新生化活性。花生皮来源于落花生，是一种用于治疗慢性出血的传统中药。我们已经表明，口服PEXT可提高小鼠外周血小板水平。最近，我们报道了一种液体培养系统，该系统可用于研究人CD34细胞的巨核细胞生成和血小板生成。在这个液体培养系统中，PEXT被证明可增强CD41/DAPI细胞（血小板）的形成，但对CD41/DAPI细胞（巨核细胞）的形成或DNA含量没有影响。此外，PEXT选择性地刺激培养的成熟巨核细胞形成前血小板，并刺激佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导Meg01细胞形成血小板样颗粒。尽管对巨核细胞集落形成单位（CFUs）的形成没有影响，但PEXT在巨核细胞从CD34细胞发育过程中增加了其大小。PEXT对GATA-1和NF-E2 mRNA水平没有影响，已知这两种基因在血小板生成中起重要作用，并且根据pMARE-Luc（pGL3-MARE-荧光素酶）分析结果，对Meg01细胞中的NF-E2激活没有影响。这些结果表明，PEXT可加速巨核细胞形成前血小板，但不影响造血干细胞向巨核细胞的发育。
