Brezovska Katerina, Panovska Ana Poceva, Grozdanova Aleksandra, Suturkova Ljubica, Basta Ivana, Apostolski Slobodan
Faculty of Pharmacy, University Ss. Cyril and Methodius, Skopje, Macedonia.
J Neurosci Rural Pract. 2011 Jul;2(2):125-9. doi: 10.4103/0976-3147.83576.
Antibodies to ganglioside GM1 are associated with Guillain-Barré Syndrome (GBS) in patients with serologic evidence of a preceding infection with Campylobacter jejuni. Molecular mimicry between C. jejuni Lipopolysaccharide (LPS) and ganglioside GM1 has been proven to be the immunopathogenic mechanism of the disease in the axonal variant of GBS. GM1-positive sera cross-react with several Gal-GalNAc-bearing glycoproteins from the human peripheral nerve and C. jejuni (O:19). This study aimed to examine the immunoreactivity of the digested cross-reactive glycoproteins isolated from the human peripheral nerve and C. jejuni (O:19) with Peanut Agglutinin (PNA) as a marker for the Gal-GalNAc determinant, and with sera from patients with GBS.
For this purpose, the cross-reactive glycoproteins from peripheral nerve and C. jejuni (O:19) were enzymatically digested with trypsin and the obtained peptides were incubated with PNA and GBS sera.
Western blot analysis of the separated peptides revealed several bands showing positive reactivity to PNA and to sera from patients with GBS, present in both digests from peripheral nerve and C. jejuni (O:19).
These data indicate the possible molecular mimicry between the cross-reactive glycoproteins present in C. jejuni and human peripheral nerve and its potential role in the development of GBS following infection with C. jejuni (O:19).
空肠弯曲菌前驱感染血清学证据阳性的吉兰-巴雷综合征（GBS）患者中，抗神经节苷脂GM1抗体与之相关。空肠弯曲菌脂多糖（LPS）与神经节苷脂GM1之间的分子模拟已被证明是GBS轴索型疾病的免疫致病机制。GM1阳性血清与人外周神经及空肠弯曲菌（O:19）的几种含Gal-GalNAc糖蛋白发生交叉反应。本研究旨在以花生凝集素（PNA）作为Gal-GalNAc决定簇的标志物，检测从人外周神经和空肠弯曲菌（O:19）中分离出的经消化的交叉反应糖蛋白与PNA及GBS患者血清的免疫反应性。
为此，用胰蛋白酶对来自外周神经和空肠弯曲菌（O:19）的交叉反应糖蛋白进行酶消化，并将所得肽段与PNA和GBS血清孵育。
对分离出的肽段进行蛋白质印迹分析显示，在外周神经和空肠弯曲菌（O:19）的两种消化产物中均出现了几条对PNA和GBS患者血清呈阳性反应的条带。
这些数据表明空肠弯曲菌和人外周神经中存在的交叉反应糖蛋白之间可能存在分子模拟，及其在空肠弯曲菌（O:19）感染后GBS发病中的潜在作用。
