Instituto de Estructura de la Materia, Consejo Superior de Investigaciones Científicas, Serrano, 121, 28006 Madrid, Spain.
Nanoscale. 2011 Oct 5;3(10):4247-53. doi: 10.1039/c1nr10551e. Epub 2011 Sep 7.
NIR-dye encoded gold nanoparticles (GNP) are rapidly emerging as contrast agents in many bio-imaging/sensing applications. The coding process is usually carried out without control or a clear understanding of the metal-liquid interface properties which, in contrast, are critical in determining the type and extension of dye-metal interaction. In this paper, we investigated the effect of gold surface composition on the adsorption of indocyanine green (ICG) on GNP, simulating the surface conditions of gold nanorods on citrate-capped gold nanospheres. These substrates allowed a careful control of the metal-liquid interface composition and, thus, detailed absorption and fluorescence concentration studies of the effects of each individual chemical in the colloidal solution (i.e. bromide anions, cetyl trimethylammonium ions and Ag(+) ions) on the ICG-gold interaction. This study reveals the drastic effect that these experimental parameters can have on the ICG adsorption on GNP.
近红外染料编码金纳米粒子（GNP）在许多生物成像/传感应用中迅速成为对比剂。编码过程通常是在没有控制或对金属-液相界面性质的清晰理解的情况下进行的，而这些性质对于确定染料-金属相互作用的类型和程度至关重要。在本文中，我们研究了金表面成分对金纳米粒子上吲哚菁绿（ICG）吸附的影响，模拟了金纳米棒在柠檬酸包覆的金纳米球上的表面条件。这些基底允许仔细控制金属-液相界面的组成，从而可以详细研究胶体溶液中每个单独化学物质（即溴化物阴离子、十六烷基三甲基铵离子和 Ag(+)离子）对 ICG-金相互作用的影响，以及它们对 ICG 吸附到 GNP 上的影响。这项研究揭示了这些实验参数对 ICG 在 GNP 上吸附的巨大影响。
