Department of Oncology, First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Rd, Zhengzhou, 450003, People's Republic of China.
Pathol Oncol Res. 2012 Apr;18(2):343-8. doi: 10.1007/s12253-011-9450-3. Epub 2011 Sep 7.
Large-scale profiling approaches have revealed global down-regulation of microRNAs (miRNAs) in several human cancer types including breast cancer. Altered expression of Dicer and Drosha, two key enzymes in the miRNA maturation, is believed to be one of the most important mechanisms. By using quantitative real-time RT-PCR (QT-PCR), we examined the expression of Dicer and Drosha in 49 pairs of matched human breast cancer tissues. Decreased expression was observed in 53.1% (Dicer), 51.9% (Drosha) and 75.5% (Dicer plus Drosha) breast cancer tissues. In conclusion, the decreased expression of Dicer and Drosha may play a role in down-regulation of miRNAs in breast cancer.
大规模分析方法显示，包括乳腺癌在内的几种人类癌症类型中的 microRNAs (miRNAs) 呈全球性下调。Dicer 和 Drosha 的表达改变，这两种 miRNA 成熟的关键酶之一，被认为是最重要的机制之一。通过使用定量实时 RT-PCR (QT-PCR)，我们检测了 49 对匹配的人乳腺癌组织中 Dicer 和 Drosha 的表达。在乳腺癌组织中观察到 53.1%（Dicer）、51.9%（Drosha）和 75.5%（Dicer 加 Drosha）的表达降低。综上所述，Dicer 和 Drosha 的表达降低可能在乳腺癌中 miRNA 的下调中发挥作用。
