Kwon Sun Young, Lee Jae-ho, Kim Bora, Park Jong-Wook, Kwon Taeg Kyu, Kang Sun Hee, Kim Shin
Department of Pathology, School of Medicine, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, South Korea.
Pathol Oncol Res. 2014 Jul;20(3):697-705. doi: 10.1007/s12253-014-9750-5. Epub 2014 Feb 27.
Altered expression of microRNA (miRNA) machinery components may play an important role in breast cancer progression. The objective of the current study was to evaluate Drosha, the DiGeorge syndrome critical region gene 8 (DGCR8), Dicer, and Argonaute 2 (AGO2) mRNA expression in invasive breast carcinoma (IBC) and to assess the value of clinical parameters on their expression. By using quantitative real-time PCR, we examined the expression of the four miRNA machinery components in 52 breast tumor tissues which are diagnosed as invasive ductal carcinoma and adjacent non-neoplastic tissues. In the present study, decreased mRNA expression levels of major miRNA machinery components were observed in IBC. The altered mRNA expression levels of DGCR8 and AGO2 are positively correlated with to each other. This study revealed for the first time that expression alterations of DGCR8 are significantly associated with estrogen receptor and Ki-67 status in IBC. Moreover, AGO2 mRNA expression level was significantly correlated with N stage. These results provided evidences that down-regulated the four miRNA machinery components may play an important role in breast pathobiology and that DGCR8 and AGO2 might be associated with important clinical factors.
微小RNA（miRNA）机制成分的表达改变可能在乳腺癌进展中起重要作用。本研究的目的是评估Drosha、迪格奥尔格综合征关键区域基因8（DGCR8）、Dicer和Argonaute 2（AGO2）在浸润性乳腺癌（IBC）中的mRNA表达，并评估临床参数对其表达的价值。通过定量实时PCR，我们检测了52例被诊断为浸润性导管癌的乳腺肿瘤组织及相邻非肿瘤组织中四种miRNA机制成分的表达。在本研究中，IBC中主要miRNA机制成分的mRNA表达水平降低。DGCR8和AGO2的mRNA表达水平改变彼此呈正相关。本研究首次揭示，DGCR8的表达改变与IBC中的雌激素受体和Ki-67状态显著相关。此外，AGO2 mRNA表达水平与N分期显著相关。这些结果提供了证据，表明四种miRNA机制成分的下调可能在乳腺病理生物学中起重要作用，并且DGCR8和AGO2可能与重要临床因素相关。
