Feurtado J Allan, Kermode Allison R
Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, SK, Canada.
Methods Mol Biol. 2011;773:53-64. doi: 10.1007/978-1-61779-231-1_4.
Many seeds of coniferous species display a deep primary dormancy at maturity and require several weeks of pretreatment to produce seed populations that germinate in a vigorous and timely manner. Facilitating an efficient transition from dormancy to germination by devising improved protocols for dormancy breakage is not only important to conifer seed research, aiding in the study of the dormancy process itself, but is also of interest and applicability to commercial forest nursery operations. In the forests of British Columbia, Canada, several conifer species are well-adapted to their environment, with seeds needing to experience long durations in the moist state at cool or fluctuating temperatures. These include yellow-cedar (Callitropsis nootkatensis), western white pine (Pinus monticola), and true fir species, such as Pacific silver fir and subalpine fir (Abies amabilis and A. lasiocarpa, respectively). In this chapter, we discuss the development of new dormancy-breaking protocols for the aforementioned species that centre on the balance of several key aspects: (1) reducing the time needed to terminate dormancy in the seed population; (2) synchronicity of germination; (3) ease of use; (4) cost-effectiveness; and (5) repeatability. Where possible, any new or modified protocol should be further tested in relationship to promoting rapid seedling growth in a forest nursery greenhouse setting and after planting at natural stands. Based on the five criteria listed above, very significant improvements compared to traditional dormancy-breaking methods have been achieved for the targeted conifer species. Where tested (e.g. yellow-cedar), the modified dormancy-breaking treatments result in vigorous growth in the greenhouse and after planting at natural stands.
许多针叶树种的种子在成熟时表现出深度的初始休眠,需要数周的预处理才能产生能够迅速且适时发芽的种子群体。通过设计改进的打破休眠方案来促进从休眠到发芽的有效转变,不仅对针叶树种子研究很重要,有助于休眠过程本身的研究,而且对商业森林苗圃运营也具有重要意义和适用性。在加拿大不列颠哥伦比亚省的森林中,几种针叶树种很好地适应了它们的环境,其种子需要在凉爽或波动的温度下在湿润状态下经历较长时间。这些树种包括黄杉(Callitropsis nootkatensis)、西部白松(Pinus monticola)以及真冷杉树种,如太平洋银冷杉和亚高山冷杉(分别为Abies amabilis和A. lasiocarpa)。在本章中,我们讨论了针对上述树种开发新的打破休眠方案,该方案围绕几个关键方面的平衡展开:(1)减少种子群体终止休眠所需的时间;(2)发芽的同步性;(3)易用性;(4)成本效益;(5)可重复性。只要有可能,任何新的或改进的方案都应在促进森林苗圃温室环境中以及在自然林分种植后幼苗快速生长方面进行进一步测试。基于上述五个标准,与传统的打破休眠方法相比,针对目标针叶树种已取得了非常显著的改进。在经过测试的地方(如黄杉),改良后的打破休眠处理在温室中以及在自然林分种植后都能促进植株茁壮成长。