Research Center for Chirotechnology, Fourth Military Medical University, Xi'an, PR China.
J Sep Sci. 2011 Sep;34(18):2455-62. doi: 10.1002/jssc.201100228. Epub 2011 Aug 23.
A novel chiral selector, clindamycin succinate, was synthesized and first used as a chiral selector in capillary electrophoresis (CE). The chiral resolution ability of this kind of clindamycin derivation was studied by CE using some racemic drugs as model analytes. From the experimental results, it was found that both resolution and selectivity of the selector were dependent on the following parameters: concentration of chiral selectors, pH of the running buffer, temperature of the capillary column, applied voltage and organic modifier used. The results show that the chiral selector possesses high resolution toward some racemic drugs, including ofloxacin, chlorphenamine, tryptophan, propranolol, sotalol and metoprolol. Excellent chiral resolution of these tested drugs was achieved under the optimal conditions of 50 mM clindamycin succinate, 10% MeOH v/v, 50 mM Tris buffer, pH 4.0, at 22 kV and 20 °C within 25 min.
一种新型的手性选择剂，克林霉素琥珀酸，被合成并首次用于毛细管电泳（CE）中的手性选择剂。通过 CE 使用一些外消旋药物作为模型分析物研究了这种克林霉素衍生物的手性拆分能力。从实验结果发现，选择剂的分辨率和选择性都取决于以下参数：手性选择剂的浓度、运行缓冲液的 pH 值、毛细管柱的温度、施加的电压和使用的有机溶剂。结果表明，该手性选择剂对一些外消旋药物具有高分辨率，包括氧氟沙星、氯苯那敏、色氨酸、普萘洛尔、索他洛尔和美托洛尔。在最佳条件下，即 50mM 克林霉素琥珀酸、10%甲醇 v/v、50mMTris 缓冲液、pH 值 4.0、22kV 和 20°C 下，在 25 分钟内即可实现这些测试药物的出色手性拆分。
