Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul University, Beyazit, Istanbul, Turkey.
J Sep Sci. 2011 Oct;34(19):2645-9. doi: 10.1002/jssc.201100489. Epub 2011 Aug 24.
A sensitive, selective, simple and fast HPLC method based on the formation of derivative with fluorescamine was developed for the determination of memantine (ME) in human plasma. Separation was achieved on a CN column (200 mm×4.6 mm) using acetonitrile-10 mM orthophosphoric acid containing 1 mL/L triethylamine (45:55, v/v) at a flow rate of 1 mL/min. Emission and excitation wavelengths were 480 and 380 nm, respectively. Amantadine was used as an internal standard. Calibration graphs were rectilinear over the range of 1.0-100.0 ng/mL. Limit of detection and limit of quantification were found to be 0.3 and 1.0 ng/mL, respectively. Intra-day and inter-day relative standard deviation values were found to be <2.03%. Average recovery was also found to be around 94%. Proposed method was applied for the pharmacokinetic study in a healthy volunteer after a single oral administration of 20 mg of ME.
建立了一种灵敏、选择性强、简单、快速的 HPLC 法，采用荧光胺衍生化法，用于测定人血浆中盐酸美金刚（ME）的浓度。采用 CN 柱（200mm×4.6mm），以乙腈-10mM 正磷酸（含 1mL/L 三乙胺，45:55，v/v）为流动相，流速为 1mL/min，在激发波长 380nm、发射波长 480nm 下检测。金刚烷胺为内标。1.0-100.0ng/mL 范围内线性关系良好，检测限和定量限分别为 0.3ng/mL 和 1.0ng/mL。日内和日间相对标准偏差均小于 2.03%。平均回收率约为 94%。该方法用于 20mg 盐酸美金刚单剂量口服给药后健康志愿者的药代动力学研究。
