电感耦合等离子体质谱法测定生物样品中的汞
[Determination of Hg in biological samples by inductively coupled plasma mass spectrometry].
作者信息
Ma Dong, Zhang Dan, Zhuo Xian-Yi, Liu Wei, Shen Bao-Hua, Shen Min
机构信息
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
出版信息
Fa Yi Xue Za Zhi. 2011 Jun;27(3):193-5.
OBJECTIVE
To establish an inductively coupled plasma mass spectrometry (ICP-MS) method for determination of Hg in biological samples.
METHODS
The samples were digested with microwave digestion instrument. ICP-MS was applied to detect Hg in blood, urine and hair specimens by using 115In as an internal marker. The ability of gold to eliminate the memory effect of mercury was investigated with the gold amalgamate produced by gold and mercury.
RESULTS
The limits of detection were in the 0.01 microg/L, and the accuracy of the method ranged from 97.0% to 107.1%. The concentration of gold was 10 microg/L and the memory effect of mercury was resolved.
CONCLUSION
The method is accurate, rapid, sensitive and suitable for the cases of mercury poisoning and the clinical diagnosis and monitoring for patients with mercury poisoning.
目的
建立电感耦合等离子体质谱法（ICP-MS）测定生物样品中的汞。
方法
采用微波消解仪对样品进行消解。以115In作为内标，应用ICP-MS检测血液、尿液和毛发标本中的汞。利用金与汞生成的金汞齐研究金消除汞记忆效应的能力。
结果
检测限为0.01μg/L，方法准确度在97.0%至107.1%之间。金浓度为10μg/L时，汞的记忆效应得到消除。
结论
该方法准确、快速、灵敏，适用于汞中毒病例及汞中毒患者的临床诊断与监测。
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