College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
J Pharm Pharmacol. 2011 Oct;63(10):1327-35. doi: 10.1111/j.2042-7158.2011.01337.x. Epub 2011 Aug 8.
In the present study, we aimed to examine the anti-atopic properties of bile from the cat fish, Silurus asotus, to determine its possible use as a pharmaceutical product.
The anti-atopic activities of cat fish bile were examined in a non-cell antioxidant, in-vitro assay (splenocytes and mast cells) and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model.
The results of these experiments revealed that Silurus asotus bile (SAB) scavenges radicals and protects proteins from superoxide attacks, suggesting that SAB suppresses the T helper (Th) type 2-skewed immune response. Th1/Th2 mRNA cytokines (interleukin (IL)-2, interferon (IFN)-γ and IL-4) from mouse splenocytes were effectively inhibited, and the release of β-hexosaminidase in RBL-2H3 mast cells was significantly suppressed by SAB. These results were supported by screening the Th1/Th2 cytokine mRNAs (IL-2, IFN-γ and IL-4) from lymph nodes in DNCB-treated mice. More dramatic results were observed in the histological changes at higher SAB concentrations (5%) compared to the therapeutic control, visualized using hematoxylin-eosin (H&E) staining.
The results presented in this study suggest that SAB may provide functional advantages with regard to treating atopic dermatitis because of its antioxidant and immune-suppressive effects.
本研究旨在考察鲶鱼胆汁的抗变态反应特性，以期将其开发为一种药物。
采用非细胞抗氧化剂、体外（脾细胞和肥大细胞）和 2,4-二硝基氯苯（DNCB）诱导的特应性皮炎样小鼠模型检测鲶鱼胆汁的抗变态反应活性。
实验结果表明，鲶鱼胆汁（SAB）能清除自由基并保护蛋白质免受超氧自由基攻击，提示 SAB 抑制辅助性 T 细胞（Th）2 型偏倚免疫反应。SAB 能有效抑制小鼠脾细胞 Th1/Th2 细胞因子（白细胞介素（IL）-2、干扰素（IFN）-γ 和 IL-4）mRNA 的表达，并显著抑制 RBL-2H3 肥大细胞β-己糖胺酶的释放。这些结果得到了 DNCB 处理小鼠淋巴结中 Th1/Th2 细胞因子（IL-2、IFN-γ 和 IL-4）mRNA 筛选结果的支持。与治疗对照相比，在较高浓度（5%）的 SAB 下观察到的组织学变化更为明显，苏木精-伊红（H&E）染色可见。
本研究结果表明，SAB 可能因其抗氧化和免疫抑制作用而在治疗特应性皮炎方面具有功能优势。
