Tichý E, Zabka M, Gardavská K, Halenárová A, Scheerová Z Kontseková, Potúcková M
Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
Pharmazie. 2011 Aug;66(8):560-3.
The poor solubility of astaxanthin in water can cause problems during dissolution tests of dosage forms because they are usually performed in water-based media. The aim of this study was the development of a convenient dissolution medium and a method for a spectrophotometric determination of astaxanthin in an aqueous solution. Three surfactants in different concentrations were tested as solubility-improving substances: sodium lauryl sulfate (SLS), polysorbate 80 (PS 80) and macrogolglycerol hydroxystearate (Cremophor RH 40, CR 40). Optimal conditions were determined. The dissolution of astaxanthin from solid dosage form is performed into 1000 g of a solution of sodium lauryl sulfate with the concentration 1.0% (w/w) at 37 degrees C by paddle method, 100 rotations per minute, dissolution time 30 minutes. The procedure is convenient for solid dosage forms with a content of 4 to 12 mg of astaxanthin. The spectrophotometric determination of astaxanthin in aqueous solution from the dissolution test is measured at 486 nm. The specific absorbance A(1%) 1cm for astaxanthin in water is 2000, a sodium lauryl sulfate solution (1%) was used as a blank.
虾青素在水中的溶解度较低，这可能会在剂型的溶出度测试过程中引发问题，因为溶出度测试通常在水基介质中进行。本研究的目的是开发一种便捷的溶出介质以及一种用于分光光度法测定水溶液中虾青素的方法。测试了三种不同浓度的表面活性剂作为增溶物质：十二烷基硫酸钠（SLS）、聚山梨酯80（PS 80）和聚乙二醇氢化蓖麻油（聚氧乙烯氢化蓖麻油RH 40，CR 40）。确定了最佳条件。将固体剂型中的虾青素在37℃下，通过桨法以每分钟100转的转速、30分钟的溶出时间，溶出到1000 g浓度为1.0%（w/w）的十二烷基硫酸钠溶液中。该方法适用于虾青素含量为4至12 mg的固体剂型。溶出度测试水溶液中虾青素的分光光度法测定在486 nm波长下进行。虾青素在水中的比吸光系数A（1%）1cm为2000，以十二烷基硫酸钠溶液（1%）作为空白对照。
