Kahn H J, Thorner P S
Department of Pathology, Women's College Hospital, Toronto, Ontario, Canada.
Pediatr Pathol. 1990;10(3):375-84. doi: 10.3109/15513819009067125.
Langerhans cells and their pathologic counterparts can be identified in paraffin sections using immunohistochemical staining for S-100 protein. This procedure is useful in confirming a diagnosis of Langerhans cell histiocytosis (LCH). However, many other cell types are also positive for S-100 protein. Positive staining for CD1 (Leu 6) supports a diagnosis of LCH, but requires frozen tissue. A panel of antibodies would be desirable in confirming a diagnosis of LCH, particularly if these antibodies could be used on paraffin-embedded material. We studied the pattern of staining for commercially available monoclonal antibodies MT1, MT2, MB2, and LN1, which were originally marketed as lymphocyte markers, using paraffin-embedded tissue sections of cases of LCH. In all 20 cases pathologic Langerhans cells stained positively with MT1 only. Various other S-100 protein-positive lesions were also examined with MT1 and were consistently negative for MT1. Other cutaneous histiocytic and mast cell lesions were positive with MT1, but S-100 protein negative. Our results demonstrate that the monoclonal antibody MT1 serves as an additional marker for LCH and, together with S-100 protein, would make up a diagnostic panel of antibodies for LCH to be used on routine paraffin-embedded sections.
使用针对S - 100蛋白的免疫组织化学染色，可在石蜡切片中识别朗格汉斯细胞及其病理对应物。该方法有助于确诊朗格汉斯细胞组织细胞增多症（LCH）。然而，许多其他细胞类型对S - 100蛋白也呈阳性反应。CD1（Leu 6）阳性染色支持LCH的诊断，但需要冷冻组织。在确诊LCH时，使用一组抗体将是可取的，特别是如果这些抗体可用于石蜡包埋材料。我们使用LCH病例的石蜡包埋组织切片，研究了最初作为淋巴细胞标志物销售的市售单克隆抗体MT1、MT2、MB2和LN1的染色模式。在所有20例病例中，病理性朗格汉斯细胞仅对MT1呈阳性染色。还用MT1检查了各种其他S - 100蛋白阳性病变，结果MT1始终为阴性。其他皮肤组织细胞和肥大细胞病变对MT1呈阳性，但S - 100蛋白为阴性。我们的结果表明，单克隆抗体MT1可作为LCH的一种额外标志物，与S - 100蛋白一起，可构成用于常规石蜡包埋切片的LCH诊断抗体组。
