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[Construction of the gene library of symbiotic nitrogen-fixing Rhizobium lupini].

作者信息

Ivanushkin A G, Marchenko G N, Chistoserdov A Iu, Pushkin A V, Kretovich V L

出版信息

Prikl Biokhim Mikrobiol. 1990 Jan-Feb;26(1):117-20.

PMID:2190206
Abstract

The gene bank of the symbiotic nitrogen-fixing bacterium Rhizobium lupini (effective strain 359a) was constructed on plasmid pAYC31 that was used to transform Escherichia coli C6000. The bank contains 6600 clones. Restriction analysis showed that the size of the mean insertion fragment in the plasmid in 6.5 kb.

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