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全关节置换术中重复使用冲洗盆是否是细菌污染的一个来源?

Is repetitive intraoperative splash basin use a source of bacterial contamination in total joint replacement?

作者信息

Glait Sergio A, Schwarzkopf Ran, Gould Steven, Bosco Joseph, Slover James

机构信息

Department of Orthopedic Surgery, NYU Hospital for Joint Diseases, New York, New York, USA.

出版信息

Orthopedics. 2011 Sep 9;34(9):e546-9. doi: 10.3928/01477447-20110714-06.

Abstract

Splash basins are used in arthroplasty cases to wash instruments. Several studies in the literature have shown these basins being a potential source of bacterial infection. This study assesses the risk of contamination of intraoperative splash basins used to wash and store instruments. A total of 46 random clean primary arthroplasty cases (32 hips, 13 knees, and 1 unicondylar knee) were studied by taking cultures of sterile splash basins as soon as they are opened (controls) and again at wound closure after instruments and debris have come into contact with the sterile water. All cultures were taken with sterile culture swabs and sent to the laboratory for aerobic, anaerobic, and fungal culture. Outcome measured was any positive culture. A total of 92 cultures from 46 cases were tested. Only 1 (2.17%) control culture, which grew Streptococcus viridans, was positive for bacterial growth. One of 46 samples (2.17%) taken at wound closure was positive for coagulase-negative Staphylococcus. Mean time between basin opening and wound closure was 180±45 minutes. For the 1 infected sample taken at the conclusion of the case, it was 240 minutes. Previous studies show contamination rates as high as 74% for splash basins used intraoperatively. Our study contradicts the belief that splash basins are a high source of infection, with only 2.17% of basins showing contamination. Splash basins can be a potential source of contamination, but the risk is not as high as previously cited in the orthopedic literature.

摘要

冲洗盆用于关节置换手术中清洗器械。文献中的多项研究表明,这些盆是细菌感染的潜在来源。本研究评估了用于清洗和存放器械的术中冲洗盆的污染风险。通过在无菌冲洗盆打开后立即采集样本(对照组),以及在器械和碎屑与无菌水接触后伤口闭合时再次采集样本,对总共46例随机的初次关节置换清洁手术病例(32例髋关节、13例膝关节和1例单髁膝关节)进行了研究。所有样本均使用无菌培养拭子采集,并送往实验室进行需氧菌、厌氧菌和真菌培养。测量的结果是任何阳性培养结果。对46例病例的总共92份样本进行了检测。只有1份(2.17%)对照组样本培养出草绿色链球菌,呈细菌生长阳性。在伤口闭合时采集的46份样本中有1份(2.17%)凝固酶阴性葡萄球菌呈阳性。盆打开至伤口闭合的平均时间为180±45分钟。对于病例结束时采集的1份感染样本,时间为240分钟。先前的研究表明,术中使用的冲洗盆污染率高达74%。我们的研究与冲洗盆是高感染源这一观点相矛盾,只有2.17%的盆显示有污染。冲洗盆可能是一个潜在的污染源,但风险并不像骨科文献中先前引用的那么高。

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