Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
Am J Reprod Immunol. 2011 Dec;66(6):544-53. doi: 10.1111/j.1600-0897.2011.01067.x. Epub 2011 Sep 8.
The VEGF-1154G>A polymorphism has been reported to be a genetic risk factor for recurrent spontaneous abortion in various studies; however, these studies have focused on genetic analyses of pregnant women rather than aborted fetuses. To evaluate and confirm the association between the VEGF-1154G>A polymorphism and spontaneous abortion, we focused on the relationship between four polymorphisms in the VEGF gene (-2578C>A, -1154G>A, -634G>C, and 936C>T) and spontaneously aborted fetuses (SAFs).
The subjects included 118 SAFs at <20 weeks gestation and 380 normal controls consisting of children and adults. The polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis.
Spontaneously aborted fetuses exhibited significantly different frequencies of the -2578CA+AA/-634CC and -1154GA+AA/-634CC combined genotypes compared with control subjects. The frequency of the -2578A/-1154A/-634C/936C haplotype was significantly higher in SAFs.
VEGF genes -2578CA+AA/-634CC and -1154GA+AA/-634CC in the fetus are possible risk factors for spontaneous abortion.
在不同的研究中，VEGF-1154G>A 多态性已被报道为复发性自然流产的遗传危险因素；然而，这些研究主要集中在对孕妇而不是流产胎儿的基因分析上。为了评估和证实 VEGF-1154G>A 多态性与自然流产之间的关联，我们关注了 VEGF 基因中的四个多态性（-2578C>A、-1154G>A、-634G>C 和 936C>T）与自然流产胎儿（SAFs）之间的关系。
研究对象包括 118 例妊娠 <20 周的 SAFs 和 380 例由儿童和成人组成的正常对照组。采用聚合酶链反应-限制性片段长度多态性分析方法对多态性进行基因分型。
与对照组相比，自然流产胎儿的 -2578CA+AA/-634CC 和 -1154GA+AA/-634CC 联合基因型的频率明显不同。SAFs 中 -2578A/-1154A/-634C/936C 单倍型的频率明显更高。
胎儿 VEGF 基因-2578CA+AA/-634CC 和 -1154GA+AA/-634CC 可能是自然流产的危险因素。
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