Pienaar Ronel, Potgieter Fred T, Latif Abdalla A, Thekisoe Oriel M M, Mans Ben J
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa.
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Private Bag X13, Phuthaditjhaba, 9866, South Africa.
Parasitology. 2011 Dec;138(14):1935-44. doi: 10.1017/S0031182011001454. Epub 2011 Sep 9.
Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites. We describe the Hybrid II assay, a real-time hybridization PCR method, which compares well with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.
走廊病是一种由适应水牛的小泰勒虫引起的牛急性致命疾病。这是南非的一种国家控制疾病，对水牛的移动实施严格的控制措施，其中包括对小泰勒虫和其他受控疾病的存在进行强制检测。使用官方实时杂交PCR检测法（Sibeko等人，2008年）对水牛中携带小泰勒虫的状态进行准确诊断，已证明会受到与类泰勒虫（水牛）寄生虫同时感染的影响。我们描述了杂交II检测法，这是一种实时杂交PCR方法，在特异性和灵敏度方面与官方杂交检测法相当。然而，它不受类泰勒虫（水牛）寄生虫混合感染的影响，因此是对当前杂交检测法的重大改进。
