Christensen Jeff, Gerdts Cory J, Clifton Mathew C, Stewart Lance
Emerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1022-6. doi: 10.1107/S1744309111023232. Epub 2011 Aug 13.
The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.
MPCS 插塞式晶体生成仪是一种基于微毛细管的蛋白质结晶系统，用于从纳升体积的蛋白质中生成适合衍射分析的晶体。使用插塞式晶体生成仪进行结晶筛选，作为一种补救途径，用于那些在使用传统坐滴气相扩散法的初始观察期内未能结晶的蛋白质。此外，用于存储由插塞式晶体生成仪设置的结晶实验的 CrystalCards 被证明是一种可行的容器，可长期存储蛋白质晶体，且随着时间推移衍射质量没有明显损失。已证明将插塞式晶体生成仪用于 SSGCID 蛋白质是一种有效的晶体挽救和存储方法。
