Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey.
Med Oncol. 2012 Sep;29(3):1928-34. doi: 10.1007/s12032-011-0057-z. Epub 2011 Sep 9.
This study was conducted to investigate the association of genetic polymorphisms in the MnSOD and GPX1 genes with the risk and invasiveness of bladder cancer in a Turkish population. This prospectively designed study enrolled 157 patients with bladder cancer (mean age 63.2 ± 10.86 years) and 224 healthy controls (mean age 61.7 ± 8.39 years). Genotyping of the MnSOD Ala-9Val and GPX1 Pro198Leu polymorphisms was carried out by PCR-RFLP. No significant difference was found in MnSOD genotype distributions between the controls and the bladder cancer patients. However, the Leu/Leu genotype of GPX1 was associated with a significantly higher risk of bladder cancer than the Pro/Pro genotype. When stratified according to tumor stage, the Leu/Leu genotype of GPX1 was more frequently observed in bladder cancer patients with high-stage tumors than those with low-stage tumors. Additionally, patients carrying both Ala/Ala of MnSOD and Leu/Leu of GPX1 had the highest risk of developing bladder cancer. In conclusion, the present study indicates that the GPX1 Pro198Leu polymorphism may be associated with the risk and development of invasive bladder cancer. In addition, the combination of the MnSOD Ala/Ala and GPX1 Leu/Leu genotypes may have a synergistic effect on disease risk.
本研究旨在探讨土耳其人群 MnSOD 和 GPX1 基因遗传多态性与膀胱癌风险和侵袭性的关系。这项前瞻性设计的研究纳入了 157 例膀胱癌患者（平均年龄 63.2 ± 10.86 岁）和 224 例健康对照者（平均年龄 61.7 ± 8.39 岁）。通过 PCR-RFLP 对 MnSOD Ala-9Val 和 GPX1 Pro198Leu 多态性进行基因分型。在对照组和膀胱癌患者中，MnSOD 基因型分布无显著差异。然而，GPX1 的 Leu/Leu 基因型与膀胱癌的风险显著增加相关，而 Pro/Pro 基因型则无此相关性。按肿瘤分期分层后，高分期膀胱癌患者中 GPX1 的 Leu/Leu 基因型比低分期患者更常见。此外，同时携带 MnSOD Ala/Ala 和 GPX1 Leu/Leu 基因型的患者发生膀胱癌的风险最高。综上所述，本研究表明 GPX1 Pro198Leu 多态性可能与膀胱癌的风险和侵袭性有关。此外，MnSOD Ala/Ala 和 GPX1 Leu/Leu 基因型的组合可能对疾病风险有协同作用。
