Service de Cardiologie, CHU Besançon, IFR 133, EA 3920, University of Franche-Comté, Besançon, France.
Basic Res Cardiol. 2011 Nov;106(6):1397-410. doi: 10.1007/s00395-011-0220-x. Epub 2011 Sep 9.
Endothelial colony-forming cells (ECFCs) are known to increase after acute myocardial infarction (AMI). We examined whether the presence of ECFCs is associated with preserved microvascular integrity in the myocardium at risk by reducing microvascular obstruction (MVO). We enrolled 88 patients with a first ST elevation AMI. ECFC colonies and circulating progenitor cells were characterized at admission. MVO was evaluated at 5 days and infarct size at 5 days and at 6-month follow-up by magnetic resonance imaging. ECFC colonies were detected in 40 patients (ECFC(pos) patients). At 5 days, MVO was of greater magnitude in ECFC(neg) versus ECFC(pos) patients (7.7 ± 5.3 vs. 3.2 ± 5%, p = 0.0002). At 6 months, in ECFC(pos) patients, there was a greater reduction in infarct size (-32.4 ± 33 vs. -12.8 ± 24%; p = 0.003) and a significant improvement in left ventricular (LV) volumes and ejection fraction. Level of circulating CD34+/VEGF-R2+ cells was correlated with the number of ECFC colonies (r = 0.54, p < 0.001) and relative change in infarct size (r = 0.71, p < 0.0001). The results showed that the presence of ECFC colonies is associated with reduced MVO after AMI, leading to reduced infarct size and less LV remodelling and can be considered a marker of preserved microvascular integrity in AMI patients.
内皮祖细胞（ECFCs）已知在急性心肌梗死（AMI）后会增加。我们研究了 ECFCs 的存在是否与通过减少微血管阻塞（MVO）来保持心肌中微血管的完整性有关。我们纳入了 88 例首次 ST 段抬高型 AMI 患者。入院时对 ECFC 集落和循环祖细胞进行了特征分析。通过磁共振成像在 5 天评估 MVO，在 5 天和 6 个月随访时评估梗死面积。在 40 例患者（ECFC(pos)患者）中检测到 ECFC 集落。在 5 天时，ECFC(neg)患者的 MVO 比 ECFC(pos)患者更大（7.7±5.3%比 3.2±5%，p=0.0002）。在 6 个月时，在 ECFC(pos)患者中，梗死面积的减少更大（-32.4±33%比-12.8±24%；p=0.003），左心室（LV）容积和射血分数也显著改善。循环 CD34+/VEGF-R2+细胞水平与 ECFC 集落的数量呈正相关（r=0.54，p<0.001），与梗死面积的相对变化呈正相关（r=0.71，p<0.0001）。结果表明，ECFC 集落的存在与 AMI 后 MVO 的减少有关，导致梗死面积减少，LV 重构减少，可以认为是 AMI 患者微血管完整性的标志物。
