Johnson L, Hardy V B, Martin M T
Department of Veterinary Anatomy, College of Veterinary Medicine, Texas A&M University, College Station 77843-4458.
Anat Rec. 1990 Jun;227(2):167-74. doi: 10.1002/ar.1092270205.
Nomarski optics were used to identify stages of the spermatogenic cycle of seminiferous tubules in sectioned tissue or in whole dispersed tubules and to characterize the equine spermatogenic wave. Embedded tissues were sectioned at 20 microns. Whole dispersed tubules were obtained by enzymatic digestion of thin slices of fresh testis. Dispersed tubules were fixed, dehydrated in graded levels of alcohol, infiltrated with Epon, and mounted in toto on glass slides. Stages of the spermatogenic cycle could be identified under Nomarski optics in both histologic sections and tubules mounted in toto. Stage dependent nuclear chromatic and cytoplasmic changes in spermatogonia, spermatocytes, and spermatids were evident. Spermatid development included chromatin condensation, nuclear elongation, acrosomal development from the Golgi and proacrosomic granules, migration of the annulus and mitochondrial alignment, and the transient appearance of the chromatoid body and manchette. Both nuclear and cytoplasmic details of Sertoli cells were revealed. In tubules mounted in toto, the spermatogenic wave along the length of the tubules occurred as a consecutive set of stages occupying small regions along the tubular length. The spermatogenic wave in the horse is more similar to that of humans than that of rats. The combination of enzymatic isolation of seminiferous tubules and identification of spermatogenic stages by Nomarski optics facilitates examination of the spermatogenic wave in species whose tubules are tightly bound and not easily teased apart.
利用诺马斯基光学显微镜来识别切片组织或完整分散的曲细精管中精子发生周期的阶段，并描述马的精子发生波。将包埋组织切成20微米厚的切片。通过对新鲜睾丸薄片进行酶消化获得完整分散的曲细精管。将分散的曲细精管固定，在不同浓度的酒精中脱水，用环氧树脂渗透，然后整体安装在载玻片上。在诺马斯基光学显微镜下，可以在组织学切片和整体安装的曲细精管中识别精子发生周期的阶段。精原细胞、精母细胞和精子细胞中依赖阶段的核染色质和细胞质变化很明显。精子细胞的发育包括染色质浓缩、核伸长、高尔基体和前顶体颗粒形成顶体、环的迁移和线粒体排列，以及拟染色体和袖套的短暂出现。支持细胞的核和细胞质细节都被揭示出来。在整体安装的曲细精管中，沿着曲细精管长度的精子发生波以一系列连续的阶段出现，这些阶段占据沿着曲细管长度的小区域。马的精子发生波与人类的比与大鼠的更相似。曲细精管的酶解分离和通过诺马斯基光学显微镜识别精子发生阶段的结合，便于对曲细管紧密相连且不易分开的物种的精子发生波进行检查。
