Zhang Ya, Zhang Weikai, Johnston Alexander H, Newman Tracey A, Pyykk Ilmari, Zou Jing
Department of Otolaryngology, University of Tampere, Medical school and University Hospital of Tampere, Finland.
Acta Otolaryngol. 2011 Dec;131(12):1249-56. doi: 10.3109/00016489.2011.615066. Epub 2011 Sep 12.
Cochleostomy is the most efficient approach in delivering PEG-b-PCL polymersomes (PMs) to the inner ear. PMs can be delivered to the vestibule by transtympanic injection or cochleostomy.
To evaluate the efficiency of delivering PEG-b-PCL PMs into the inner ear using different approaches.
The PEG-b-PCL PMs were administered either by sustained topical round window membrane (RWM) delivery using gelatin sponge pledgets in combination with an osmotic pump, transtympanic injection, or cochleostomy. The distribution of the PMs in the inner ear was observed by confocal microscopy using either whole mount specimens or cryosections.
Cochleostomy resulted in distribution of the PMs in the spiral ligament (SL), mesothelial cells beneath the organ of Corti, supporting cells in the organ of Corti, and spiral ganglion cells (SGCs). Transtympanic injection induced uptake of the PMs in the SL and mesothelial cells beneath the organ of Corti. Topical administration showed distribution of the PMs only in the SL. In the vestibulum, transtympanic injection and cochleostomy induced more distribution of the PMs than did topical RWM delivery (p < 0.05, Kruskal-Wallis test).
耳蜗造口术是将聚乙二醇-嵌段-聚己内酯聚合物囊泡（PMs）递送至内耳的最有效方法。PMs可通过经鼓膜注射或耳蜗造口术递送至前庭。
评估使用不同方法将聚乙二醇-嵌段-聚己内酯PMs递送至内耳的效率。
聚乙二醇-嵌段-聚己内酯PMs通过使用明胶海绵小块结合渗透泵进行持续局部圆窗膜（RWM）给药、经鼓膜注射或耳蜗造口术给药。使用整装标本或冰冻切片通过共聚焦显微镜观察PMs在内耳中的分布。
耳蜗造口术导致PMs分布于螺旋韧带（SL）、柯蒂氏器下方的间皮细胞、柯蒂氏器中的支持细胞以及螺旋神经节细胞（SGCs）。经鼓膜注射诱导PMs被SL和柯蒂氏器下方的间皮细胞摄取。局部给药显示PMs仅分布于SL。在前庭中，经鼓膜注射和耳蜗造口术比局部RWM给药诱导更多的PMs分布（p < 0.05，Kruskal-Wallis检验）。
