[卡介苗处理后人外周血单个核细胞的Toll样受体4表达及其免疫激活作用]

[TLR4 expression of human PBMC treated by BCG and its role of immune activation].

作者信息

Sun Rui, Yang Xiong, Sun Er-Lin, Han Rui-Fa

机构信息

Based Medical Research Center, Tianjin Medical University, Tianjin 300070, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Sep;27(9):945-8.

PMID:21906464

Abstract

AIM

To investigate TLR4 expression of human peripheral blood mononuclear cells (hPBMC) treated with BCG and its role of immune activation.

METHODS

hPBMC were treated with BCG in vitro. TLR4 expression were detected by flow cytometry, IFN-γ and TNF-α expression of hPBMC in both BCG stimulated group and the control group were detected by ELISA.

RESULTS

The expression of TLR4 in hPBMC treated with BCG was stronger than the control group significantly (P<0.01) and increased with the time. In 72 h the TLR4 expression of BCG group was (44.73 ± 0.0066)%, while the control group was (1.02 ± 0.0024)%. BCG can promote hPBMC proliferation, and this enhancement was time-dependent. In 24 h, 48 h and 72 h IFN-γ and TNF-α expression of BCG group were significantly higher than the control group(P<0.05), and this enhancement was time-dependent.

CONCLUSION

BCG can on enhance TLR4 expression and promote immune activation of hPBMC.

摘要

目的

探讨卡介苗处理后人外周血单个核细胞（hPBMC）中Toll样受体4（TLR4）的表达及其免疫激活作用。

方法

体外使用卡介苗处理hPBMC。采用流式细胞术检测TLR4表达，采用酶联免疫吸附测定（ELISA）法检测卡介苗刺激组和对照组hPBMC中γ干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）的表达。

结果

卡介苗处理的hPBMC中TLR4表达显著高于对照组（P<0.01），且随时间增加。72小时时，卡介苗组TLR4表达为（44.73±0.0066）%，而对照组为（1.02±0.0024）%。卡介苗可促进hPBMC增殖，且这种增强具有时间依赖性。24小时、48小时和72小时时，卡介苗组IFN-γ和TNF-α表达显著高于对照组（P<0.05），且这种增强具有时间依赖性。