Yuan Bo, He Hong-Yan, Wang Ming-Yong, Liu Jian
Department of Nephrology, Luzhou Medical College, Luzhou 646000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Sep;27(9):962-4.
To explore the influence of angiotensin-(1-7)[Ang-(1-7)] on angiotension II(Ang II) induced rat's tubular epithelial-myofibroblast transdifferentiation and the secretion of extracellular matrix.
The NRK52E were maintained and sub-cultured treated with Ang-(1-7) (10(-6); mmol/L) and Ang II(10(-6); mmol/L) for 24, 48, 72, 96 hours, we detect the protein expressions of E-cadherin and α-SMA by immunocytochemistry method; The content of Col I and FN in the cultured supernatant were measured by ELISA; The mRNA expression of E-cadherin, α-SMA, Col I and FN was detected by real-time PCR.
Treat with ang II 96 h, the protein and mRNA expression of E-cadherin decreased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN increased significantly (P<0.05); treat with Ang II and Ang-(1-7), the protein and mRNA expression of E-cadherin increased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN decreased significantly (P<0.05).
Ang-(1-7) can inhibits Ang II-induced rat's tubular epithelial myofibroblast transdifferentiation and decrease the secretion of FN and Col I.
