Wang Jiang-Tao, Wang Jie
Department of Medical Research, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Sep;27(9):979-81.
To select single-chain variable fragment(scFv) antibody specific for bone sialoprotein(BSP) from Human Single Fold scFv Libraries.
Human Single Fold scFv Libraries were panned against immobilized BSP in a microtiter plate, after three rounds of panning, 96 clones were determined specific to BSP. The specificity of each scFv clone was determined by ELISA. The coding gene for BSP protein scFv has been sequenced.
Phage antibody for BSP protein had a specific combination character. There were 368 bp, 527 bp, 935 bp which werer light chain, heavy chain and joint gene fragment with the resuLt of PCR. The DNA sequence data showed that there were 11 differences of the amino acids in the light chain, while there were only 3 differences in the heavy chain of scFv.
scFv specific to BSP has been identified by means of phage display technology.
从人单链折叠scFv文库中筛选出对骨唾液酸蛋白（BSP）具有特异性的单链可变片段（scFv）抗体。
在微量滴定板中，用人单链折叠scFv文库对固定化的BSP进行淘选，经过三轮淘选后，确定96个对BSP具有特异性的克隆。通过酶联免疫吸附测定（ELISA）确定每个scFv克隆的特异性。对BSP蛋白scFv的编码基因进行了测序。
针对BSP蛋白的噬菌体抗体具有特异性结合特性。聚合酶链反应（PCR）结果显示，轻链、重链和连接基因片段分别为368bp、527bp、935bp。DNA序列数据表明，scFv轻链中的氨基酸有11处差异，而重链中仅有3处差异。
通过噬菌体展示技术鉴定出了对BSP具有特异性的scFv。
