Wang Yong-qian, Li Xiao-feng, Zhou Xin, Liu Xin-quan, Wang Wei-ping
Department of Neurosurgery, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Zhong Xi Yi Jie He Xue Bao. 2011 Sep;9(9):991-7. doi: 10.3736/jcim20110910.
To investigate the protective effects of Zuogui Pill (ZGP), a compound Chinese herbal medicine, on retina ganglion cells (RGCs) in rats after optical nerve clipping injury.
Optic nerves in Sprague-Dawley rats were crushed intraorbitally at 2 mm behind eyeball by using a forceps with a 140-gram chucking power for 30 s to induce unilateral injured model. The injured rats were fed with ZGP (ZGP group, 4.0 g/kg body weight each day) and equal volume of normal saline (injury group) respectively. Morphological changes in the retina were observed and the RGCs were quantified in the specimens. Nestin and glial fibrillary acidic protein (GFAP) expressions in the retina were detected by immunohistochemical fluorescence staining at different time points after nerve injury.
After optical never crushing, the thickness of retina and the number of RGCs were significantly decreased in injury group as compared with the normal group. Nearly 50% of the total RGCs were disappeared at the 4th week in the injury group. The expression levels of nestin and GFAP in the retina were markedly increased after optical never crushing at the 2nd week and this lasted to the 8th week, and gradually decreased to the normal level at the 16th week. In contrast, the arrangement of retinal cells was regular in the ZGP group, and the number of survival RGCs was greater than that of the injury group. Meanwhile, the nestin and GFAP expressions in retina were significantly enhanced as compared with the injury group at all time points.
ZGP may have protective effects on rat optic nerve and RGCs after contusion by promoting the nestin and GFAP expressions in Müller cells of the retina.
探讨复方中药左归丸（ZGP）对大鼠视神经夹伤损伤后视网膜神经节细胞（RGCs）的保护作用。
采用夹力为140克的镊子在Sprague-Dawley大鼠眼球后2毫米处眶内夹伤视神经30秒，诱导单侧损伤模型。将损伤大鼠分别给予ZGP（ZGP组，每天4.0克/千克体重）和等体积生理盐水（损伤组）。观察视网膜形态学变化，并对标本中的RGCs进行定量分析。在神经损伤后的不同时间点，通过免疫组织化学荧光染色检测视网膜中巢蛋白和胶质纤维酸性蛋白（GFAP）的表达。
视神经夹伤后，损伤组视网膜厚度和RGCs数量与正常组相比显著降低。损伤组在第4周时，近50%的RGCs总数消失。视神经夹伤后第2周，视网膜中巢蛋白和GFAP的表达水平显著升高，并持续至第8周，在第16周逐渐降至正常水平。相比之下，ZGP组视网膜细胞排列规则，存活的RGCs数量多于损伤组。同时，在所有时间点，ZGP组视网膜中巢蛋白和GFAP的表达均显著高于损伤组。
ZGP可能通过促进视网膜Müller细胞中巢蛋白和GFAP的表达，对大鼠视神经挫伤及RGCs具有保护作用。
