Mishra Sanjiv K, Shrivastav Anupama, Maurya Rahulkumar R, Patidar Shailesh K, Haldar Soumya, Mishra Sandhya
Discipline of Salt Marine and Inorganic Chemicals, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), G.B. Marg, Bhavnagar 364 021, India.
Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), G.B. Marg, Bhavnagar 364 021, India.
Protein Expr Purif. 2012 Jan;81(1):5-10. doi: 10.1016/j.pep.2011.08.011. Epub 2011 Aug 28.
The isolated cyanobacterium containing biopigments like chlorophyll-a, phycoerythrin, phycocyanin, and carotenoid was cultured under different quality of light modes to ascertain biomass and pigment productivity. On the basis of 16S rRNA gene sequence, the isolate was identified as Pseudanabaena sp. Maximum biomass concentration obtained in white-, blue-, and green-light was 0.82, 0.94, and 0.89 g/L, respectively. It was observed that maximum phycoerythrin production was in green light (39.2 mg/L), ensued by blue light (32.2 mg/L), while phycocyanin production was maximum in red light (10.9 mg/L). In yellow light, pigment production as well as the growth rate gradually declined after 12 days. Carotenoid production decreased in blue-, white-, and red-light after 15 days, while in green light it had increased gradually. The present communication suggests that Pseudanabaena sp. can be used for commercial production of phycoerythrin when grown under green light.
将含有叶绿素-a、藻红蛋白、藻蓝蛋白和类胡萝卜素等生物色素的分离蓝细菌在不同光质模式下培养，以确定生物量和色素产量。基于16S rRNA基因序列，该分离株被鉴定为伪鱼腥藻属。在白光、蓝光和绿光下获得的最大生物量浓度分别为0.82、0.94和0.89 g/L。观察到藻红蛋白的最大产量在绿光下（39.2 mg/L），其次是蓝光（32.2 mg/L），而藻蓝蛋白的产量在红光下最高（10.9 mg/L）。在黄光下，12天后色素产量和生长速率逐渐下降。15天后，蓝光、白光和红光下类胡萝卜素产量下降，而绿光下类胡萝卜素产量逐渐增加。本通讯表明，伪鱼腥藻属在绿光下生长时可用于商业生产藻红蛋白。
