Laboratório de Neurobiologia da Dor e Inflamação, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, Brazil Programa de Pós-graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, Brazil Laboratório de Marcadores Histológicos, Instituto Federal de Educação, Ciência e Tecnologia de Santa Catarina, Campus Lages, Lages, SC, Brazil Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, Brazil.
Pain. 2011 Nov;152(11):2653-2661. doi: 10.1016/j.pain.2011.08.014. Epub 2011 Sep 8.
An important issue in physical rehabilitation is how to protect from or to reduce the effects of peripheral nerve injury. In the present study, we examined whether ankle joint mobilization (AJM) would reduce neuropathic pain and enhance motor functional recovery after nerve injury. In the axonotmesis model, AJM during 15 sessions every other day was conducted in rats. Mechanical and thermal hyperalgesia and motor performance deficit were measured for 5 weeks. After 5 weeks, we performed morphological analysis and quantified the immunoreactivity for CD11b/c and glial fibrillary acidic protein (GFAP), markers of glial activation, in the lumbar spinal cord. Mechanical and thermal hyperalgesia and motor performance deficit were found in the Crush+Anesthesia (Anes) group (P<0.001), which was significantly decreased after AJM (P<0.001). In the morphological analysis, the Crush+Anes group presented reduced myelin sheath thickness (P<0.05), but the AJM group presented enhanced myelin sheath thickness (P<0.05). Peripheral nerve injury increased the immunoreactivity for CD11b/c and GFAP in the spinal cord (P<0.05), and AJM markedly reduced CD11b/c and GFAP immunoreactivity (P<0.01). These results show that AJM in rats produces an antihyperalgesic effect and peripheral nerve regeneration through the inhibition of glial activation in the dorsal horn of the spinal cord. These findings suggest new approaches for physical rehabilitation to protect from or reduce the effects of nerve injury.
在物理康复中，一个重要的问题是如何预防或减轻周围神经损伤的影响。在本研究中，我们研究了踝关节活动（AJM）是否会减轻神经损伤后的神经病理性疼痛并促进运动功能恢复。在轴索切断模型中，在大鼠中进行了 15 次隔日 AJM 治疗。在 5 周内测量机械性和热痛觉过敏以及运动功能缺陷。5 周后，我们进行了形态学分析，并定量了腰椎脊髓中胶质激活标志物 CD11b/c 和胶质纤维酸性蛋白（GFAP）的免疫反应性。在 Crush+麻醉（Anes）组中发现了机械性和热痛觉过敏以及运动功能缺陷（P<0.001），AJM 后明显减轻（P<0.001）。在形态学分析中，Crush+Anes 组的髓鞘厚度降低（P<0.05），但 AJM 组的髓鞘厚度增加（P<0.05）。周围神经损伤增加了脊髓中 CD11b/c 和 GFAP 的免疫反应性（P<0.05），AJM 显著降低了 CD11b/c 和 GFAP 的免疫反应性（P<0.01）。这些结果表明，AJM 在大鼠中通过抑制脊髓背角中的胶质激活产生抗痛觉过敏作用和周围神经再生。这些发现为物理康复提供了新的方法，以预防或减轻神经损伤的影响。
