Reproductive Endocrinology and Cell Signaling Laboratory, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Mol Cell Endocrinol. 2012 Jan 2;348(1):211-23. doi: 10.1016/j.mce.2011.08.020. Epub 2011 Aug 31.
Prostaglandin E2 (PGE(2)) plays pleiotropic roles at fetal-maternal interface during establishment of pregnancy. The objectives of the study were to: (i) determine regulation of PGE2 receptors EP1, EP2, EP3, and EP4 in the endometrium during the estrous cycle and early pregnancy; and (ii) understand endometrial epithelial and stromal cell-specific hormonal regulation of EP2 and EP4 in sheep. Results indicate that: (i) early pregnancy induces expression of EP2 and EP4 but not EP1 and EP3 proteins in the endometrium on days 12-16 compared to that of estrous cycle; (ii) intrauterine infusion of interferon tau (IFNT) increases expression of EP2 and EP4 proteins in endometrium; and (iii) IFNT activates distinct epithelial and stromal cell-specific JAK, EGFR, ERK1/2, AKT, or JNK signaling module to regulate expression of EP2 and EP4 proteins in the ovine endometrium. Our results indicate a role for EP2 and EP4-mediated PGE(2) signaling in endometrial functions and establishment of pregnancy in ruminants.
前列腺素 E2（PGE（2））在妊娠建立期间在胎儿-母体界面发挥多效作用。本研究的目的是：（i）确定发情周期和早期妊娠期间子宫内膜中 PGE2 受体 EP1、EP2、EP3 和 EP4 的调节；（ii）了解绵羊子宫内膜上皮和基质细胞对 EP2 和 EP4 的激素特异性调节。结果表明：（i）与发情周期相比，妊娠早期（第 12-16 天）诱导子宫内膜中 EP2 和 EP4 的表达，但不诱导 EP1 和 EP3 蛋白的表达；（ii）子宫内输注干扰素 tau（IFNT）增加子宫内膜中 EP2 和 EP4 蛋白的表达；（iii）IFNT 激活不同的上皮和基质细胞特异性 JAK、EGFR、ERK1/2、AKT 或 JNK 信号模块，以调节绵羊子宫内膜中 EP2 和 EP4 蛋白的表达。我们的结果表明 EP2 和 EP4 介导的 PGE（2）信号在反刍动物的子宫内膜功能和妊娠建立中起作用。
