Department of Functional Biochemistry of Nervous System, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia.
Epilepsy Behav. 2011 Nov;22(3):433-41. doi: 10.1016/j.yebeh.2011.08.002. Epub 2011 Sep 9.
There are increasing data on the influence of seizures on neurogenesis in the adult brain. However, data on cell proliferation and differentiation during the early stages of kindling are scarce. We have used pentylenetetrazole (PTZ)-induced kindling to investigate the temporal profile of cytogenesis in the germinative zones of adult rat brain. For comparison, we also used a single PTZ-induced generalized tonic-clonic seizure. During kindling development, the density of 5-bromo-2'-deoxyuridine-positive cells demonstrated similar changes in all germinative zones: a dramatic decrease after the first subthreshold PTZ injection, and a gradual increase to the control level following repeated PTZ administration. On the contrary, a single PTZ-induced generalized tonic-clonic seizure was followed by an increase in the number of proliferating cells in both the dentate gyrus and the subventricular zone. These results may indicate the existence of global mechanisms affecting cellular proliferation in adult brain during seizures. Different temporal profiles of neuronal damage and proliferation changes suggest that neurodegeneration is unlikely to be a global proliferation-regulating factor. The data may contribute to better understanding of the initial phase of kindling development and epileptogenesis.
越来越多的数据表明，癫痫发作会影响成年人大脑的神经发生。然而，关于点燃早期细胞增殖和分化的数据却很少。我们使用戊四氮（PTZ）诱导的点燃来研究成年大鼠脑生发区细胞发生的时间进程。为了进行比较，我们还使用了单次 PTZ 诱导的全面强直-阵挛性发作。在点燃发展过程中，所有生发区的 5-溴-2'-脱氧尿苷阳性细胞密度均表现出相似的变化：第一次亚阈值 PTZ 注射后急剧下降，随后在重复 PTZ 给药后逐渐增加至对照水平。相反，单次 PTZ 诱导的全面强直-阵挛性发作后，齿状回和侧脑室下区的增殖细胞数量增加。这些结果可能表明，在癫痫发作期间，存在影响成年人大脑细胞增殖的全局机制。神经元损伤和增殖变化的不同时间进程表明，神经退行性变不太可能是一个全局的增殖调节因素。这些数据可能有助于更好地理解点燃发展和癫痫发生的初始阶段。
