Nanofabrication Systems Division, NanoInk, Skokie, IL 60077, USA.
Anal Biochem. 2011 Dec 15;419(2):339-41. doi: 10.1016/j.ab.2011.08.021. Epub 2011 Aug 18.
Tip-based direct protein printing is a relatively new technique that is useful for controlling the cellular microenvironment with subcellular resolution. Coculture studies have been useful for mimicking the in vivo environment and studying effects on stem or progenitor cell function. However, there are many experimental variables that cannot be properly controlled and may lead to confounding results. Here we demonstrate a technique that allows spatial control of multiple cell types at single cell levels on a substrate. Specifically, 3T3 fibroblasts and C2C12 myoblasts and their respective binding dynamics with fibronectin and laminin demonstrate the single cell coculture concept.
基于尖端的直接蛋白质打印是一种相对较新的技术，可用于亚细胞分辨率控制细胞微环境。共培养研究对于模拟体内环境和研究对干细胞或祖细胞功能的影响很有用。然而，有许多实验变量无法得到适当控制，这可能会导致结果混淆。在这里，我们展示了一种技术，该技术可在底物上的单细胞水平上对多种细胞类型进行空间控制。具体来说，3T3 成纤维细胞和 C2C12 成肌细胞及其与纤连蛋白和层粘连蛋白的各自结合动力学证明了单细胞共培养的概念。
