Laboratório de Ultraestrutura Celular Hertha Meyer, Universidade Federal do Rio de Janeiro, Brazil.
Micron. 2012 Feb;43(2-3):494-6. doi: 10.1016/j.micron.2011.08.008. Epub 2011 Aug 22.
Giardia duodenalis is a human parasite that colonises the small intestine. In some studies, it may be necessary to estimate the parasite-host adhesion index. When working in vitro, it is very difficult to determine the relative number of parasites adhered to intestinal cells because parasites might be removed from enterocytes during specimen preparation. We have encountered such difficulties with sample analyses when using light and conventional scanning electron microscopy, including the loss of adherent cells during the processing steps. In this study, we showed that environmental scanning electron microscopy (ESEM), which allows the examination of specimens at normal atmospheric pressure or in very low vacuum without any previous treatment, can be used effectively in quantitative studies of the parasite-host relationship.
十二指肠贾第鞭毛虫是一种寄生在小肠内的人体寄生虫。在某些研究中，可能需要估计寄生虫-宿主黏附指数。在体外工作时，由于寄生虫可能在标本制备过程中从肠细胞中脱落，因此很难确定黏附在肠细胞上的寄生虫相对数量。在使用光学和常规扫描电子显微镜进行样本分析时，我们遇到了这样的困难，包括在处理步骤中黏附细胞的丢失。在这项研究中，我们表明，环境扫描电子显微镜（ESEM）可以在无需任何预处理的情况下，在正常大气压或极低真空下检查标本，可有效地用于寄生虫-宿主关系的定量研究。
