Corbett Erik M, Grooms Daniel L, Bolin Steven R, Bartlett Ben, Grotelueschen Dale M
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, 165 NFSTC Building, Michigan State University, East Lansing, MI 48824, USA.
J Vet Diagn Invest. 2011 May;23(3):511-5. doi: 10.1177/1040638711403412.
Forty-seven cattle management groups from 36 herds in a regional Bovine viral diarrhea virus (BVDV) eradication program were selected to evaluate serology as a tool to detect herd infection with BVDV. Serum samples were obtained from 5 non-vaccinated sentinel calves ≥ 6 months old in each management group and virus neutralizing (VN) antibody titers against BVDV genotypes 1 and 2 were determined. A herd was considered positive if 2 or more sentinel calves had VN antibody titers ≥ 128 to either genotype. Results were compared to individual animal testing of all available calves by reverse transcription polymerase chain reaction (RT-PCR) on skin biopsy samples. In 1 management group from 1 herd (n = 24), 3 sentinel calves had VN antibody titers ≥ 128. Three ear notch samples from that herd were positive for BVDV on RT-PCR assay. All other management groups were negative for BVDV. In the present study, the herd sensitivity of sentinel serology was 100% (95% confidence interval [CI]: 0.05-1.0) and herd specificity was 100% (95% CI: 0.90-1.0). The κ value for agreement between sentinel serology and RT-PCR was 1.0 (95% CI: 1.0-1.0). Preliminary results suggest that sentinel animal serology can be utilized in a BVDV eradication program to provide an accurate and efficient evaluation of herd status.
在一项区域性牛病毒性腹泻病毒（BVDV）根除计划中，从36个牛群的47个牛管理组中选取样本，以评估血清学作为检测牛群感染BVDV工具的效果。从每个管理组中5头年龄≥6个月的未接种疫苗的哨兵犊牛采集血清样本，并测定针对BVDV 1型和2型的病毒中和（VN）抗体效价。如果2头或更多哨兵犊牛针对任一基因型的VN抗体效价≥128，则该牛群被视为阳性。将结果与通过对所有可用犊牛的皮肤活检样本进行逆转录聚合酶链反应（RT-PCR）的个体动物检测结果进行比较。在来自1个牛群（n = 24）的1个管理组中，3头哨兵犊牛的VN抗体效价≥128。该牛群的3个耳缺刻样本在RT-PCR检测中BVDV呈阳性。所有其他管理组的BVDV检测均为阴性。在本研究中，哨兵血清学的牛群敏感性为100%（95%置信区间[CI]：0.05 - 1.0），牛群特异性为100%（95% CI：0.90 - 1.0）。哨兵血清学与RT-PCR之间的一致性κ值为1.0（95% CI：1.0 - 1.0）。初步结果表明，哨兵动物血清学可用于BVDV根除计划，以准确、高效地评估牛群状况。
