Gagnon Carl A, Tremblay Josée, Larochelle Danielle, Music Nedzad, Tremblay Donald
Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte Street, St-Hyacinthe, Quebec, Canada.
J Vet Diagn Invest. 2011 Sep;23(5):986-90. doi: 10.1177/1040638711418615.
On December 8, 2008, a male fisher (Martes pennanti) housed in a quarantine enclosure at the St-Félicien Zoo was found dead with multiple skin ulcers on the muzzle and plantar pads. At necropsy, no major findings were found, and a specific cause of death was not determined microscopically. However, at the borders of ulcerated sites, there were increased numbers of koilocytes, with perinuclear vacuolation and nuclear enlargement. A pan-herpesvirus nested polymerase chain reaction (PCR) assay was conducted, and an expected PCR product of 230 nucleotides was obtained within tissues collected from around the skin ulcers. Other tissues, including intestines and pool of lung, liver, and kidney, tested negative. The obtained PCR amplicon was sequenced and was highly related to the partial viral DNA polymerase (DPOL) gene of Mustelid herpesvirus 1. Virus isolation was negative, and no virion was detected by electron microscopy. The pathogenic potential of this novel herpesvirus and its role in the death of the fisher are unknown.
2008年12月8日，圣费利西安动物园检疫围栏内的一只雄性渔貂（Martes pennanti）被发现死亡，口鼻部和脚垫上有多处皮肤溃疡。尸检时未发现重大异常，显微镜检查也未确定具体死因。然而，在溃疡部位的边缘，空泡化细胞数量增加，伴有核周空泡化和核肿大。进行了泛疱疹病毒巢式聚合酶链反应（PCR）检测，在从皮肤溃疡周围采集的组织中获得了预期的230个核苷酸的PCR产物。其他组织，包括肠道以及肺、肝和肾的混合样本，检测结果均为阴性。对获得的PCR扩增子进行测序，结果显示其与鼬科疱疹病毒1的部分病毒DNA聚合酶（DPOL）基因高度相关。病毒分离结果为阴性，电子显微镜检查未检测到病毒粒子。这种新型疱疹病毒的致病潜力及其在渔貂死亡中的作用尚不清楚。
