Department of Biology and Genetics, Medical University of Lublin, 20-093, Lublin, Chodźki 4A, Poland.
Cell Mol Biol Lett. 2011 Dec;16(4):610-24. doi: 10.2478/s11658-011-0026-8. Epub 2011 Sep 7.
This study aimed to determine the changes in the expression of genes for selected specific transcriptional factors that have both activating and repressing functions in in vitro ductal breast cancer cells, under the influence of paclitaxel, applying the microarray technique. The cells are treated with 60 ng/ml and 300 ng/ml doses of paclitaxel that correspond to those applied in breast cancer therapy. About 60 ng/ml doses of paclitaxel cause a statistically significant increase in expression of all the 16 analysed genes coding transcriptional factors, ranging from 1.84-fold (for PO4F2) to 4.65-fold (for LMO4) (p < 0.05) in comparison with the control cells, and enhanced the taxane mechanism of action. The 300 ng/ml doses of paclitaxel cause a cytotoxic effect in the cells. In this article, we argue that these changes in gene expression values may constitute prognostic and predictive factors in ductal breast cancer therapy.
本研究旨在应用微阵列技术确定紫杉醇作用于体外乳腺癌细胞时，具有激活和抑制功能的特定转录因子基因表达的变化。用分别相当于乳腺癌治疗中应用剂量的 60ng/ml 和 300ng/ml 的紫杉醇处理细胞。约 60ng/ml 的紫杉醇剂量引起 16 个分析的编码转录因子的基因的表达均显著增加，范围从 1.84 倍（PO4F2）至 4.65 倍（LMO4）（p < 0.05），与对照细胞相比，并增强了紫杉烷的作用机制。300ng/ml 的紫杉醇剂量对细胞产生细胞毒性作用。在本文中，我们认为这些基因表达值的变化可能构成导管乳腺癌治疗的预后和预测因素。
