整合素LFA-1的无细胞配体结合分析
Cell-free ligand-binding assays for integrin LFA-1.
作者信息
Yuki Koichi
机构信息
Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston, Boston, MA, USA.
出版信息
Methods Mol Biol. 2012;757:73-8. doi: 10.1007/978-1-61779-166-6_6.
Integrin LFA-1 plays an important role in leukocyte trafficking to inflammatory sites as well as the interaction of T cells with antigen-presenting cells. Inhibition of the LFA-1:ICAM-1 interactions is a promising therapeutic approach for alleviating inflammation and autoimmune diseases. This chapter describes cell-free assays to study the LFA-1:ICAM-1 interactions. These assays may be used for screening novel LFA-1 antagonists.
整合素淋巴细胞功能相关抗原-1（LFA-1）在白细胞向炎症部位的迁移以及T细胞与抗原呈递细胞的相互作用中发挥着重要作用。抑制LFA-1与细胞间黏附分子-1（ICAM-1）的相互作用是缓解炎症和自身免疫性疾病的一种有前景的治疗方法。本章介绍了用于研究LFA-1与ICAM-1相互作用的无细胞检测方法。这些检测方法可用于筛选新型LFA-1拮抗剂。
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